The mechanism of vault opening from the high resolution structure of the N-terminal repeats of MVP

@article{QuerolAudi2009TheMO,
  title={The mechanism of vault opening from the high resolution structure of the N-terminal repeats of MVP},
  author={Jordi Querol-Audí and Arnau Casa{\~n}as and Isabel Us{\'o}n and Daniel Luque and Jos{\'e} R. Cast{\'o}n and Ignasi Fita and N{\'u}ria Verdaguer},
  journal={The EMBO Journal},
  year={2009},
  volume={28},
  pages={3450 - 3457}
}
Vaults are ubiquitous ribonucleoprotein complexes involved in a diversity of cellular processes, including multidrug resistance, transport mechanisms and signal transmission. The vault particle shows a barrel‐shaped structure organized in two identical moieties, each consisting of 39 copies of the major vault protein MVP. Earlier data indicated that vault halves can dissociate at acidic pH. The crystal structure of the vault particle solved at 8 Å resolution, together with the 2.1‐Å structure… 
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32 Citations
Background Citations
5
Methods Citations
1

32 Citations

Structural Dynamics of the Vault Ribonucleoprotein Particle
TLDR
The high resolution, crystal structure of the of the seven N-terminal domains of MVP, forming the central vault barrel, revealed the interactions governing vault association and suggested a pH-dependent mechanism for a reversible dissociation induced by low pH.
Structural studies of large nucleoprotein particles, vaults
Vault is the largest nonicosahedral cytosolic nucleoprotein particle ever described. The widespread presence and evolutionary conservation of vaults suggest important biologic roles, although their…
Symmetry disruption commits vault particles to disassembly
TLDR
The molecular determinants for the vault opening process are characterized using a combination of vault recombinant reconstitution and structural techniques to inform a hitherto uncharacterized step of the vault cycle and will aid current engineering efforts leveraging vault for tailored cargo delivery.
Solution Structures of Engineered Vault Particles
TLDR
Vaults obtained by engineering at the N terminus of rat major vault protein an HIV-1 Gag protein segment are determined and their near-atomic resolution structures are determined in a solution/non-crystalline environment.
Bacterial Major Vault Protein homologs shed new light on origins of the enigmatic organelle
TLDR
It is speculated that the most likely scenario for vault appearance in eukaryotes is horizontal gene transfer from cyanobacteria, and it was shown that MVP sequences from chemotrophic bacteria Myxococcales and Cytophagales contain a domain homologous to Eukaryotic band-7 domain, unlike cyanobacterial and eukARYotic major vault proteins.
The Vault Nanoparticle: A Gigantic Ribonucleoprotein Assembly Involved in Diverse Physiological and Pathological Phenomena and an Ideal Nanovector for Drug Delivery and Therapy
TLDR
A better understanding of the physiological roles of this ribonucleoproteic complex may help develop new therapeutic strategies capable of coping with cancer progression and extend the scope of its exploitation as a nanocarrier for drug delivery.
New features of vault architecture and dynamics revealed by novel refinement using the deformable elastic network approach.
TLDR
Re-refinement of the vault structure by incorporating the high-resolution information available for the R1-7 domains, using the deformable elastic network (DEN) approach and maintaining strict 39-fold noncrystallographic symmetry is reported.
Direct visualization of vaults within intact cells by electron cryo-tomography
TLDR
These studies confirm that intracellular vaults are similar in overall size and shape to purified and recombinant vaults previously analyzed, and observe that vaults located in the extreme periphery of the cytoplasm predominately associate with granule-like structures and actin.
Structure of internalin InlK from the human pathogen Listeria monocytogenes.
Mechanical stability and reversible fracture of vault particles.
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References

SHOWING 1-10 OF 40 REFERENCES
Solution structure of a two-repeat fragment of major vault protein.
The Structure of Rat Liver Vault at 3.5 Angstrom Resolution
TLDR
The x-ray structure of rat liver vault is determined at 3.5 angstrom resolution and it is shown that the cage structure consists of a dimer of half-vaults, with eachhalf-vault comprising 39 identical major vault protein (MVP) chains.
Structural domains of vault proteins: a role for the coiled coil domain in vault assembly.
TLDR
This study identified and analyzed structural domains involved in vault assembly with emphasis on protein-protein interactions and demonstrated within MVP an intramolecular binding site and show that MVP molecules interact with each other via their coiled coil domain.
Cryoelectron microscopy imaging of recombinant and tissue derived vaults: localization of the MVP N termini and VPARP.
Structure of the vault, a ubiquitous celular component.
Structural stability of vault particles.
TLDR
EM imaging showed the opening of intact vaults into flowerlike structures when transitioning from neutral to acidic pH, which has potential use in the development of recombinant vault into nanocapsules for drug delivery since one mechanism by which therapeutic agents entrapped in vaults could be released is through an opening of the intact vault structure.
Assembly of Vault-like Particles in Insect Cells Expressing Only the Major Vault Protein*
TLDR
These particles are the first example of a single protein polymerizing into a non-spherically, non-cylindrically symmetrical structure and will enable us to design agents that disrupt vault formation and aid in elucidating vault functionin vivo.
Vaults and the major vault protein: Novel roles in signal pathway regulation and immunity
TLDR
The current knowledge on MVP and vaults is reviewed with focus on regulatory functions in intracellular signal transduction and immune defence and the definition of precise vault functions is reviewed.
Vaults. III. Vault ribonucleoprotein particles open into flower-like structures with octagonal symmetry
TLDR
A structural model is proposed that predicts the stoichiometry of vault proteins and RNA, defines vault dimer-monomer interactions, and describes two possible modes for unfolding of vaults into flowers, likely to play a role in vault function.
Preliminary analysis of two and three dimensional crystals of vault ribonucleoprotein particles.
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