The mechanism of type IA topoisomerases.

@article{Dekker2002TheMO,
  title={The mechanism of type IA topoisomerases.},
  author={Nynke H Dekker and Valentin V Rybenkov and Michel Duguet and Nancy J Crisona and Nicholas R. Cozzarelli and David Bensimon and Vincent Croquette},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99 19},
  pages={12126-31}
}
The topology of cellular DNA is carefully controlled by enzymes called topoisomerases. By using single-molecule techniques, we monitored the activity of two type IA topoisomerases in real time under conditions in which single relaxation events were detected. The strict one-at-a-time removal of supercoils we observed establishes that these enzymes use an enzyme-bridged strand-passage mechanism that is well suited to their physiological roles and demonstrates a mechanistic unity with type II… CONTINUE READING

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