The mechanism of alpha-ketoisocaproate oxygenase. Formation of beta-hydroxyisovalerate from alpha-ketoisocaproate.

@article{Sabourin1982TheMO,
  title={The mechanism of alpha-ketoisocaproate oxygenase. Formation of beta-hydroxyisovalerate from alpha-ketoisocaproate.},
  author={P J Sabourin and Loran L. Bieber},
  journal={The Journal of biological chemistry},
  year={1982},
  volume={257 13},
  pages={7468-71}
}
A soluble alpha-ketoisocaproate oxygenase from rat liver catalyzes the decarboxylation and hydroxylation of alpha-ketoisocaproate to form beta-hydroxyisovalerate. The source of oxygen (O2 or H2O) enzymatically incorporated into beta-hydroxyisovalerate was investigated using 18O2 and H218O. Greater than 92% of the carboxyl groups of beta-hydroxyisovaleric acid contained 1 18O atom from 18O2 and 15% of the beta-hydroxyl oxygens of beta-hydroxyisovaleric acid contained 18O from 18O2. Since some… CONTINUE READING