The mechanism of activation of IRAK1 and IRAK4 by interleukin-1 and Toll-like receptor agonists

@inproceedings{Vollmer2017TheMO,
  title={The mechanism of activation of IRAK1 and IRAK4 by interleukin-1 and Toll-like receptor agonists},
  author={Stefan Vollmer and Sam Strickson and Tinghu Zhang and Nathanael S Gray and Katherine L Lee and Vikram R Rao and Philip S. Cohen},
  booktitle={The Biochemical journal},
  year={2017}
}
We have developed the first assays that measure the protein kinase activities of interleukin-1 receptor-associated kinase 1 (IRAK1) and IRAK4 reliably in human cell extracts, by employing Pellino1 as a substrate in conjunction with specific pharmacological inhibitors of IRAK1 and IRAK4. We exploited these assays to show that IRAK4 was constitutively active and that its intrinsic activity towards Pellino1 was not increased significantly by stimulation with interleukin-1 (IL-1) in IL-1R… CONTINUE READING
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