The mechanism of action of the Pseudomonas aeruginosa-encoded type III cytotoxin, ExoU.

@article{Sato2003TheMO,
  title={The mechanism of action of the Pseudomonas aeruginosa-encoded type III cytotoxin, ExoU.},
  author={Hiromi Sato and Dara W. Frank and Cecilia J. Hillard and Jimmy B. Feix and Ravi R Pankhaniya and Kiyoshi Moriyama and Viviane Finck-Barbançon and Adam H Buchaklian and Ming Lei and Roy M. Long and Jeanine P. Wiener-Kronish and Teiji Sawa},
  journal={The EMBO journal},
  year={2003},
  volume={22 12},
  pages={2959-69}
}
Pseudomonas aeruginosa delivers the toxin ExoU to eukaryotic cells via a type III secretion system. Intoxication with ExoU is associated with lung injury, bacterial dissemination and sepsis in animal model and human infections. To search for ExoU targets in a genetically tractable system, we used controlled expression of the toxin in Saccharomyces cerevisiae. ExoU was cytotoxic for yeast and caused a vacuolar fragmentation phenotype. Inhibitors of human calcium-independent (iPLA(2)) and… CONTINUE READING