The mechanism of DNA replication primer synthesis by RNA polymerase

@article{Zenkin2006TheMO,
  title={The mechanism of DNA replication primer synthesis by RNA polymerase},
  author={Nikolay Zenkin and Tatyana Naryshkina and Konstantin Kuznedelov and Konstantin Severinov},
  journal={Nature},
  year={2006},
  volume={439},
  pages={617-620}
}
RNA primers for DNA replication are usually synthesized by specialized enzymes, the primases. However, some replication systems have evolved to use cellular DNA-dependent RNA polymerase for primer synthesis. The main requirement for the replication primer, an exposed RNA 3′ end annealed to the DNA template, is not compatible with known conformations of the transcription elongation complex, raising a question of how the priming is achieved. Here we show that a previously unrecognized kind of… CONTINUE READING

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