The mechanism mediating regenerative intercellular Ca2+ waves in the blowfly salivary gland

@article{Zimmermann1999TheMM,
  title={The mechanism mediating regenerative intercellular Ca2+ waves in the blowfly salivary gland},
  author={B. Zimmermann and B. Walz},
  journal={The EMBO Journal},
  year={1999},
  volume={18}
}
Intercellular Ca2+ signaling in intact salivary glands of the blowfly Calliphora erythrocephala was studied by fluorimetric digital imaging combined with microinjection of putative messenger molecules. Iontophoretic injection of D‐myo‐inositol 1,4,5‐trisphosphate (InsP3) into salivary gland cells evoked regenerative intercellular Ca2+ waves that spread through the impaled cell and several rows of surrounding cells. Ca2+ increases induced by microinjection of Ca2+ ions were confined to the… Expand
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References

SHOWING 1-10 OF 65 REFERENCES
Calcium store depletion activates two distinct calcium entry pathways in secretory cells of the blowfly salivary gland.
TLDR
The results indicate that Ca2+ store depletion by 5-HT or thapsigargin activates two distinct store-operated Ca2- entry pathways, one of which supports tonic [Ca2+]i increases and the other is transiently activated. Expand
Serotonin‐induced intercellular calcium waves in salivary glands of the blowfly Calliphora erythrocephala.
TLDR
Digital calcium‐imaging experiments on Blowfly salivary glands are consistent with the hypothesis that Ca2+ acts as the intercellular messenger and modulates its own release via positive and negative feedback on the inosital 1,4,5‐trisphosphate (InsP3) receptor. Expand
Roles of Ca2+, inositol trisphosphate and cyclic ADP-ribose in mediating intercellular Ca2+ signaling in sheep lens cells.
TLDR
Ca2+, IP3 and cADPR can all mediate intercellular Ca2+ waves by passing through gap junction channels, but both IP3 or cAD PR are more effective inter cellular messengers than Ca2+. Expand
Intercellular propagation of calcium waves mediated by inositol trisphosphate.
TLDR
It is demonstrated that the release ofCa2+ by IP3 is necessary for the propagation of intercellular Ca2+ waves and suggested that IP3 moves through gap junctions to communicate inter cellular Ca 2+ waves. Expand
Inositol trisphosphate is required for the propagation of calcium waves in Xenopus oocytes.
TLDR
Heparin, which inhibits the binding of InsP3 to its receptor, prevented the migration of Ca2+ waves induced by a poorly metabolized InsP 3 (InsP3S3), which suggested that Ca2- waves move through the cell via the serial release of Ca1+ from InsP2-sensitive stores. Expand
Coordinated intercellular calcium waves induced by noradrenaline in rat hepatocytes: dual control by gap junction permeability and agonist
TLDR
It is found that second messengers and [Ca2+]i rises in one hepatocyte cannot trigger Ca2+ responses in connected cells, suggesting that diffusion across gap junctions, while required for coordination, is not sufficient by itself for the propagation of intercellular Ca2+, waves. Expand
Molecular mechanisms of intracellular calcium excitability in X. laevis oocytes
TLDR
A model for intracellular Ca2+ signaling is proposed in which wave propagation is controlled by IP3-mediated Ca 2+ release from internal stores, but is modulated by the cytoplasmic concentration and diffusion of Ca2+. Expand
Coordination of Ca2+ Signaling by Intercellular Propagation of Ca2+ Waves in the Intact Liver (*)
TLDR
It is concluded that intercellular Ca2+ waves may provide a mechanism to coordinate responses across the functional units of the liver. Expand
Intercellular calcium waves in rat pancreatic acini: mechanism of transmission.
TLDR
Gap-junctional communication may be an important factor in amplifying a threshold signal produced in one cell throughout the acinus, resulting in enhanced stimulated secretion in acinar preparations compared with preparations of isolated cells. Expand
Mechanical stimulation and intercellular communication increases intracellular Ca2+ in epithelial cells.
TLDR
The hypothesis that IP3 acts as a cellular messenger that mediates communication through gap junctions between ciliated epithelial cells is supported. Expand
...
1
2
3
4
5
...