The measurement of serum transferrin by iron-binding capacity

  title={The measurement of serum transferrin by iron-binding capacity},
  author={W. N. M. Ramsay},
  journal={Journal of Clinical Pathology},
  pages={691 - 696}
  • W. Ramsay
  • Published 1 September 1973
  • Medicine
  • Journal of Clinical Pathology
Two criteria which ought to be satisfied by an acceptable method for the estimation of serum transferrin by iron-binding capacity are enunciated. A screening procedure is described which involves the sequential quantitative use of ion exchange and gel filtration. Its use in testing the extent to which two published methods fulfil the recommended criteria is described. Both methods give results for total iron-binding capacity which are 1-10% high because of the inclusion of non-transferrin iron. 
The measurement of iron-binding capacity in serum and purified transferrin with the aid of chemical affinity chromatography.
  • W. Ramsay
  • Biology
    Journal of clinical pathology
  • 1975
Validation experiments confirm that transferrin in serum and in pure solution is saturated with iron and give some evidence of specificity, and the possible use of commercially available transferrin preparations as analytical reference standards is discussed.
Use of an alumina column in estimating total iron-binding capacity.
A simple, rapid technique for saturating the transferrin in serum and then removing unbound (excess) iron using an easily prepared column of basic chromatographic alumina and a saturating solution of ferric chloride in citric acid.
The Measurement of Total and Unsaturated Iron‐Binding Capacity in Serum
  • Medicine
    British journal of haematology
  • 1978
An inter‐laboratory investigation into the assay of serum iron binding capacity by the magnesium carbonate adsorption method was undertaken and protocols for the clinical assay of UIBC and TIBC are given.
Determination of serum iron and iron binding capacity by NMR.
A T1 map of samples from healthy volunteers, prepared as in the determination of serumIron and TIBC by NMR, is presented to demonstrate the loss of paramagnetic contribution of serum iron upon addition of ascorbic acid.
Non‐Specific Serum Iron in Thalassaemia: an Abnormal Serum Iron Fraction of Potential Toxicity
Findings indicate that non‐specific iron is a chelatable compound which is readily available for transferrin binding, and its identification in thalassaemic sera might be of relevance to the pathogenesis of tissue damage and the protective effect of iron chelating therapy in this disease.
A simple and sensitive catalytic method for determination of the total iron-binding capacity of blood serum
SummaryA catalytic method is proposed for determination of the total iron-binding capacity of blood serum, based on the iron-catalysed oxidation ofp-phenetidine by hydrogen peroxide. Only 0.05 ml of
Ferritin and iron studies in anaemia and chronic disease
This review will primarily focus on iron deficiency anaemia and anaemia of chronic disease; however, iron overload in anaemia will also be briefly discussed.
Vergleichende Bestimmung von Transferrin und EBK im Humanserum. Ein Beitrag zur Problematik der unspezifischen Eisen-Protein-Bindung
In 150 Seren stationarer Patienten ist die TEBK mit den Verfahren von Asal (Hobl KG, Schwalbach), Asid (Asid-Institut, Munchen), Haury (Dr. Haury, Munchen), Hyland (Ferro-Check-II, Travenol,


Problems in the measurement of iron binding capacity in serum.
Method for the determination of unsaturated iron-binding capacity of serum using radioactive iron and magnesium carbonate
The radioactive iron-magnesium carbonate method was shorter and simpler and equally reproducible than the methods of Caraway, Herbert, Gottlieb, Lau, Fisher, Grevirtz, and Wasserman (1966), and Bothwell, Jacobs, and Kamener (1959).
Problems in quality control in determinations of serum total iron-binding capacity by the magnesium carbonate method.
With serum from patients the amount of magnesium carbonate is not critical but with some commercial control sera theamount of iron removed from the serum/fert ic chloride mixture is dependent on the number of magnesium Carbonate added (Table I).
An evaluation of adsorption methods for measurement of plasma iron-binding capacity.
  • J. Cook
  • Medicine
    The Journal of laboratory and clinical medicine
  • 1970
It is concluded that light MgCO 3 and coated charcoal are equally valid for determining the iron-binding capacity; however, light M gCO 3 is preferable because it is readily available, requires no preparation, and can be used interchangeably in isotopic and colorimetric assays.
A Gel Filtration Method for Determining Total Iron-Binding Capacity in Serum
  • I. Nielsen
  • Chemistry, Medicine
    Zeitschrift fur klinische Chemie und klinische Biochemie
  • 1968
The method has the advantage that it utilizes the whole specimen thus requiring smaller serum samples, and incorporates phenol as a deproteinising agent producing optically clear supernatants.
  • B. Davis
  • Medicine
    Annals of the New York Academy of Sciences
  • 1964
The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Proposed recommendations for measurement of serum iron in human blood.
  • Medicine
  • 1971