The measurement of oxidative damage to DNA by HPLC and GC/MS techniques.

  title={The measurement of oxidative damage to DNA by HPLC and GC/MS techniques.},
  author={Barry Halliwell and Miral Dizdaroğlu},
  journal={Free radical research communications},
  volume={16 2},
Oxidative damage to DNA has been measured by quantitating 8-hydroxy-2'-deoxyguanosine (8-OHdGuo) after enzymic digestion of DNA, followed by HPLC separation and electrochemical detection. Alternatively, 8-hydroxyguanine (and a wide range of other base-derived products of free radical attack) may be measured after acidic hydrolysis of DNA or chromatin, followed by derivatization and gas-chromatography/mass spectrometry. Both techniques have comparable sensitivity, but GC/MS enables determination… CONTINUE READING
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