The measurement of mitochondrial beta-oxidation by release of 3H2O from [9,10-3H]hexadecanoate: application to skeletal muscle and the use of inhibitors as models of metabolic disease.

@article{Kler1992TheMO,
  title={The measurement of mitochondrial beta-oxidation by release of 3H2O from [9,10-3H]hexadecanoate: application to skeletal muscle and the use of inhibitors as models of metabolic disease.},
  author={R. S. Kler and H. Sherratt and D. Turnbull},
  journal={Biochemical medicine and metabolic biology},
  year={1992},
  volume={47 2},
  pages={
          145-56
        }
}
We describe the use of a simple assay for beta-oxidation which depends on the release of 3H2O from [9,10-3H]hexadecanoate. This was compared with the use of [1-14C]hexadecanoate which gave comparable results when all the products of beta-oxidation were measured. The prediction that 75% of the tritium is released as 3H2O and 25% as [2-3H]acetyl units was confirmed. The assay was used successfully to demonstrate impaired beta-oxidation in tissue preparations from rats treated with etomoxir and… Expand
Control of mitochondrial beta-oxidation flux.
  • S. Eaton
  • Medicine, Biology
  • Progress in lipid research
  • 2002
A simple and rapid method to characterize lipid fate in skeletal muscle
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References

SHOWING 1-10 OF 20 REFERENCES
14CO2 production is no adequate measure of [14C]fatty acid oxidation.
Complementation analysis of fatty acid oxidation disorders.
  • A. Moon, W. Rhead
  • Biology, Medicine
  • The Journal of clinical investigation
  • 1987
...
1
2
...