The mating system of the cellular slime mould Dictyostelium discoideum

  title={The mating system of the cellular slime mould Dictyostelium discoideum},
  author={Gillian E. Robson and Keith Leslie Williams},
  journal={Current Genetics},
SummaryThe mating reaction (macrocyst formation) and vegetative compatibility (which is believed to be associated with only the mating type locus in D. discoideum) of asexual, bisexual and homothallic strains of Dictyostelium discoideum were examined. Three asexual strains were vegetatively compatible with a matA tester strain and vegetatively incompatible with a mata tester strain, so we propose that these asexual strains are in fact strains of matA mating type with defective mating capacity… 

Genetics of sex determination in the social amoebae

The mating‐type locus of the widely studied model organism Dictyostelium discoideum, which has three mating types, has recently been identified and may be a first glimpse of a novel mode of regulation used in these organisms.

Sex Determination in the Social Amoeba Dictyostelium discoideum

The discovery and analysis of the mating-type locus of the model organism Dictyostelium discoideum, a social amoeba that has three sexes that do not resemble those in any other eukaryote studied so far, is reported.

Reproductive Strategies in Social Amoeba

The sexual pathway of the social amoeba is interesting, and studies on dictyostelids will provide evolutionary insights into reproductive strategies adapted by simple multicellular organisms.

A Surface Glycoprotein Indispensable for Gamete Fusion in the Social Amoeba Dictyostelium discoideum

It is suggested that MacA is indispensable for gamete interactions in D. discoideum, probably via cell adhesion, and there is a possibility that it is controlled in a mating-type-dependent manner.

Chromosomal mapping of tRNA genes from Dictyostelium discoideum

Analysis of wild-type isolates of Dictyostelium discoideum established that both of the tRNA gene families are dispersed among sites on several of the chromosomes.

Cultivation, spore production, and mating.

Dictyostelium discoideum proliferates as solitary amoebae, constitutes multicellular structures called fruiting bodies, and mates to form macrocysts depending on environmental conditions. All of

Gamete fusion and cytokinesis preceding zygote establishment in the sexual process of Dictyostelium discoideum

It is found that the multinuclear fused cells formed during this process frequently underwent cytokinesis driven by random local movements, and that microtubule organizing centers (MTOC), abundant in the fused cells at the beginning, gradually decreased and only one of them remained within the developed macrocyst.

Heterogenic Incompatibility in Fungi

Inhibition of recombination results by a lack of karyogamy (sexual incompatibility) as well as by an inability of the nuclei to coexist in a common cytoplasm and to undergo somatic recombination (vegetative incompatibility).



Interconversion of Yeast Mating Types II. Restoration of Mating Ability to Sterile Mutants in Homothallic and Heterothallic Strains.

Observations indicate that a yeast cell contains an additional copy (or copies) of alpha information, and lead to the "cassette" model for mating type interconversion.

A genetic study of aggregation in the cellular slime mould Dictyostelium discoideum using complementation analysis.

Calculations from the results suggest a genetic complexity of about 50 genes that are specific and essential for aggregation in the cellular slime mould Dictyostelium discoideum.

Genetic exchanges in the macrocysts of Dictyostelium discoideum.

Crosses were made between strains of Dictyostelium discoideum involving two drug resistance markers and the mating-type locus, indicating that germination might be influenced by both extrinsic and multiple genetic factors.

Sexuality in the cellular slime mold Dictyostelium giganteum.

By pairing of strains of Dictyostelium giganteum in various combinations this species was shown to be heterothallic and the results support the view that the myxamoebae that emerge upon germination of the macrocysts are the products of meiosis.

The genetic system controlling homothallism in Saccharomyces yeasts.

There are four types of life cycles in Saccharomyces cerevisiae and its related species. A perfect homothallic life cycle (the Ho type) is observed in the classic D strain. Two other types show

Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. I. Isolation and phenotypic characterization of nonmating mutants.

Results strongly indicate that the sex factors are functionally involved in the conjugation process in yeast strains of both mating types.

Characterization of Dominant Resistance to Cobalt Chloride in DICTYOSTELIUM DISCOIDEUM and Its Use in Parasexual Genetic Analysis.

Hypersensitivity to cycloheximide in combination with dominant cobaltous chloride resistance provides a means of selecting diploids without the use of temperature-sensitive mutations.

The breeding system of Polysphondylium pallidum, a cellular slime mold.

A mating type analysis was performed on 231 isolates of the cellular slime mold, Polysphondylium pallidum found in 61 samples collected in eastern North America between northern Florida and sourthern Canada, suggesting that the majority of isolates represent a species capable of local genetic adaptation to a niche.

Mitotic Arrest and Chromosome Doubling Using Thiabendazole, Cambendazole, Nocodazole and Ben Late in the Slime Mould Dictyostelium discoideum

SUMMARY: Thiabendazole and cambendazole induced mitotic arrest and isogenic diploid formation through chromosome doubling in amoebae of the cellular slime mould Dictyostelium discoideum grown either

Meiosis in Dictyostelium mucoroides

Genetic confirmation is presented that in D. mucoroides (strain DM-7) the assortment of mutant traits among progeny of single macrocysts is consistent only with meiosis, suggesting karyogamy and meiosis are an integral part of dormancy and germination of this developmental stage.