The mapping by high-pH anion-exchange chromatography with pulsed amperometric detection and capillary electrophoresis of the carbohydrate moieties of human plasma alpha 1-acid glycoprotein.

@article{Hermentin1992TheMB,
  title={The mapping by high-pH anion-exchange chromatography with pulsed amperometric detection and capillary electrophoresis of the carbohydrate moieties of human plasma alpha 1-acid glycoprotein.},
  author={Peter Dr Hermentin and Rolf Witzel and Reiner D{\"o}nges and Ryan Bauer and Hansgeorg Haupt and Tanaz Patel and Raj B. Parekh and Dieter Brazel},
  journal={Analytical biochemistry},
  year={1992},
  volume={206 2},
  pages={
          419-29
        }
}
The reducing oligosaccharides released from alpha 1-acid glycoprotein (AGP) by conventional hydrazinolysis have been analyzed by two different mapping techniques, using high-pH anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) and capillary electrophoresis (CE) with uv detection at 190 nm. The CE measurements proved about 4000 times more sensitive than the measurements by HPAE-PAD. The N-glycan pool was fractionated by Mono Q anion-exchange chromatography, and… CONTINUE READING

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