The mannobiose-forming exo-mannanase involved in a new mannan catabolic pathway in Bacteroides fragilis

@article{Kawaguchi2013TheME,
  title={The mannobiose-forming exo-mannanase involved in a new mannan catabolic pathway in Bacteroides fragilis},
  author={Kazuki Kawaguchi and Takeshi Senoura and Shigeaki Ito and Toki Taira and Hiroyuki Ito and Jun Wasaki and Susumu Ito},
  journal={Archives of Microbiology},
  year={2013},
  volume={196},
  pages={17-23}
}
We have proposed a new mannan catabolic pathway in Bacteroides fragilis NCTC 9343 that involves a putative mannanase ManA in glycoside hydrolase family 26 (BF0771), a mannobiose and/or sugar transporter (BF0773), mannobiose 2-epimerase (BF0774), and mannosylglucose phosphorylase (BF0772). If this hypothesis is correct, ManA has to generate mannobiose from mannans as the major end product. In this study, the BF0771 gene from the B. fragilis genome was cloned and expressed in Escherichia coli… CONTINUE READING