The manipulation of neural and cellular activities by ectopic expression of melanopsin

@article{Koizumi2013TheMO,
  title={The manipulation of neural and cellular activities by ectopic expression of melanopsin},
  author={Amane Koizumi and Kenji F. Tanaka and Akihiro Yamanaka},
  journal={Neuroscience Research},
  year={2013},
  volume={75},
  pages={3-5}
}

The distribution of melanopsin (OPN4) protein in the human brain

The wide distribution of OPN4 in central areas of the humanbrain evokes a question whether ambient light has important straight targets in the human brain outside the retinohypothalamic tract (RHT).

Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats.

It was showed that during retinal degeneration, the expression of melanopsin was significantly decreased, while vector-mediated overexpression of melanOPSin delayed the loss of visual function in rats.

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Human melanopsin-AAV2/8 transfection to retina transiently restores visual function in rd1 mice.

Ectopic expression of human melanopsin effectively and safely restores visual function in rd1 mice and restoration of vision disappeared 45d after human melanopin injection.

Optogenetics in Developmental Biology: using light to control ion flux-dependent signals in Xenopus embryos.

Evidence is yielded that the direction of ion flux via common optogenetic reagents may be reversed, or unpredictable in non-neural cells, when used in combination with rigorous controls, and optogenetics can be a powerful tool for investigating ion-flux based signaling inNon-excitable systems.

Principles of Optogenetic Methods and Their Application to Cardiac Experimental Systems

Use of optogenetics has expanded in cardiac physiology, mainly using optically controlled depolarizing ion channels to control heart rate and for optogenetic defibrillation.

A live cell assay of GPCR coupling allows identification of optogenetic tools for controlling Go and Gi signaling

The GsX assay is presented, a set of tools based on chimeric Gs subunits that transduce coupling of opsins to diverse G proteins into increases in cAMP levels, measured with a real-time reporter in living cells to compare coupling to Gi/o/t across a panel of natural and chimeric opsins selected for potential application in gene therapy for retinal degeneration.

Prospects of Optogenetic Prosthesis of the Degenerative Retina of the Eye

The review discusses the prospects of using rhodopsin as an optogenetic tool for prosthetics of degenerative (blind) eye retina and the principles of optogenetic techniques. Retinal-containing

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