The mammary gland as a bioreactor: factors regulating the efficient expression of milk protein-based transgenes.

Abstract

Specific regulatory regions required for hormonal regulation and tissue-specific expression of rat beta-casein and why acidic protein (WAP) genes in the mammary gland have been defined. Composite response elements with multiple binding sites for several transcription factors have been identified. Mammary gland-specific gene expression appears not to be mediated by a single transcription factor, but instead requires cooperative interactions among several factors. Signal transduction pathways regulated by lactogenic hormones result in transcription factor binding and interaction within these elements, chromatin-structure changes, and milk-protein gene expression. Intragenic sequences in the 5' and 3' untranslated regions of the beta-casein and WAP mRNAs, respectively, also appear crucial for the efficient expression of these genes. Vectors to target the expression of heterologous genes, such as insulin-like growth factor I, to the mammary gland can be designed. This technology can be used to manipulate milk composition in transgenic animals, one result being improved infant formulas.

Statistics

050100'98'00'02'04'06'08'10'12'14'16
Citations per Year

298 Citations

Semantic Scholar estimates that this publication has 298 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Rosen1996TheMG, title={The mammary gland as a bioreactor: factors regulating the efficient expression of milk protein-based transgenes.}, author={Jeffrey Rosen and Samantha J. Li and Brian Raught and Darryl L . Hadsell}, journal={The American journal of clinical nutrition}, year={1996}, volume={63 4}, pages={627S-32S} }