The mammalian AMP-activated protein kinase complex mediates glucose regulation of gene expression in the yeast Saccharomyces cerevisiae.

@article{Ye2014TheMA,
  title={The mammalian AMP-activated protein kinase complex mediates glucose regulation of gene expression in the yeast Saccharomyces cerevisiae.},
  author={Tian Ye and Loubna Bendrioua and David Carmena and Ra{\'u}l Garc{\'i}a-Salcedo and Peter Dahl and David Carling and Stefan Hohmann},
  journal={FEBS letters},
  year={2014},
  volume={588 12},
  pages={2070-7}
}
The AMP-activated protein kinase (AMPK) controls energy homeostasis in eukaryotic cells. Here we expressed hetero-trimeric mammalian AMPK complexes in a Saccharomyces cerevisiae mutant lacking all five genes encoding yeast AMPK/SNF1 components. Certain mammalian complexes complemented the growth defect of the yeast mutant on non-fermentable carbon sources. Phosphorylation of the AMPK α1-subunit was glucose-regulated, albeit not by the Glc7-Reg1/2 phosphatase, which performs this function on… CONTINUE READING