The majority of G0 transgenic mice are derived from mosaic embryos

  title={The majority of G0 transgenic mice are derived from mosaic embryos},
  author={Christopher Bruce Alexander Whitelaw and Anthea J. Springbett and John Webster and John Bates Clark},
  journal={Transgenic Research},
Most transgenic mice are generated by the direct microinjection of DNA fragments into the pronuclei of fertilized eggs. It has been generally assumed that the majority of integration events occur prior to the first round of chromosomal DNA replication (Palmiter and Brinster, 1986). In this study we have determined by comparison of PCR, Southern blot and transmission frequencies that at least 62% of integration events generate a mosaic (somatic and/or germline) G0 transgenic mouse. Furthermore… CONTINUE READING

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