The major neutral proteinase of Entamoeba histolytica

  title={The major neutral proteinase of Entamoeba histolytica},
  author={W E Keene and M G Petitt and Susan K Allen and James H McKerrow},
  journal={The Journal of Experimental Medicine},
  pages={536 - 549}
FPLC anion-exchange and chromatofocusing chromatography were used to purify the major neutral proteinase from secretions of axenically cultured Entamoeba histolytica trophozoites. HM-1 strain trophozoites, which were more proteolytically active than the less virulent HK-9 strain, were used for purification of the enzyme. It is a thiol proteinase with a subunit Mr of approximately 56,000, a neutral pH optimum, and a pI of 6. The importance of this enzyme in extraintestinal amoebiasis is… CONTINUE READING


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