The maize brittle1 gene encodes amyloplast membrane polypeptides

@article{Sullivan1995TheMB,
  title={The maize brittle1 gene encodes amyloplast membrane polypeptides},
  author={Thomas D. Sullivan and Yasuko Kaneko},
  journal={Planta},
  year={1995},
  volume={196},
  pages={477-484}
}
A chimeric protein, formed of 56 amino acids from the carboxy terminus of the maize (Zea mays L.) wild-type Brittle1 (Bt1) protein fused to the glutathione-S-transferase gene, was synthesized in Escherichia coli, and used to raise antibodies. Following affinity purification, the antibodies recognized a set of 38-to 42-kDa proteins in endosperm from wild-type Bt1 plants, as well as from brittle2, shrunken2 and sugary1 plants, but not in mutant bt1 endosperm. Bt1 proteins were not detected with… CONTINUE READING

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