The luteinizing hormone receptor: influence of buffer composition on ligand binding and signaling of wild type and mutant receptors.

@article{Angelova2003TheLH,
  title={The luteinizing hormone receptor: influence of buffer composition on ligand binding and signaling of wild type and mutant receptors.},
  author={Krassimira Angelova and Prema Narayan and David Puett},
  journal={Molecular and cellular endocrinology},
  year={2003},
  volume={204 1-2},
  pages={1-9}
}
There is evidence that ligand binding to and ligand-mediated signaling by the luteinizing hormone receptor (LHR) are influenced by buffer conditions, including ionic type and strength, an issue that becomes important in comparing functional parameters obtained on receptor mutants under different conditions. In order to study this phenomenon, we performed binding (kinetic and saturation) and signaling studies of human chorionic gonadotropin (hCG) with wild type (wt) LHR and several mutants… CONTINUE READING