The low-temperature method for study of coniferous tissues in the environmental scanning electron microscope.

@article{Nedla2015TheLM,
  title={The low-temperature method for study of coniferous tissues in the environmental scanning electron microscope.},
  author={Vil{\'e}m Neděla and Eva Tihlař{\'i}kov{\'a} and Jiř{\'i} Hřib},
  journal={Microscopy research and technique},
  year={2015},
  volume={78 1},
  pages={13-21}
}
The use of non-standard low-temperature conditions in environmental scanning electron microscopy might be promising for the observation of coniferous tissues in their native state. This study is aimed to analyse and evaluate the method based on the principle of low-temperature sample stabilization. We demonstrate that the upper mucous layer is sublimed and a microstructure of the sample surface can be observed with higher resolution at lower gas pressure conditions, thanks to a low-temperature… CONTINUE READING

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