The low processivity of T7 RNA polymerase over the initially transcribed sequence can limit productive initiation in vivo.

@article{Lopez1997TheLP,
  title={The low processivity of T7 RNA polymerase over the initially transcribed sequence can limit productive initiation in vivo.},
  author={Pascal Jean Lopez and Jean Guillerez and Rui Sousa and Marc Dreyfus},
  journal={Journal of molecular biology},
  year={1997},
  volume={269 1},
  pages={41-51}
}
In vitro, after binding to the promoter to form a catalytically active complex, RNA polymerases abortively cycle over the first transcribed nucleotides (initial transcribed sequence or ITS) before leaving the promoter. With the bacteriophage T7 enzyme, the extent of abortive transcription varies with the nature of the ITS and with the elongation speed of the polymerase. Here, we compare in vitro and in vivo the yield of long transcripts from T7 promoters, with two different ITSs, the T7 gene10… CONTINUE READING
8 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-8 of 8 extracted citations

Similar Papers

Loading similar papers…