The lipopolysaccharide-induced pro-inflammatory response in RAW264.7 cells is attenuated by an unsaturated fatty acid–bovine serum albumin complex and enhanced by a saturated fatty acid–bovine serum albumin complex

@article{Chang2011TheLP,
  title={The lipopolysaccharide-induced pro-inflammatory response in RAW264.7 cells is attenuated by an unsaturated fatty acid–bovine serum albumin complex and enhanced by a saturated fatty acid–bovine serum albumin complex},
  author={Chi Fen Chang and Yat Pang Chau and Hsiu Ni Kung and Kuo Shyan Lu},
  journal={Inflammation Research},
  year={2011},
  volume={61},
  pages={151-160}
}
A 1:1 ratio of fatty acid (FA)–albumin complex was chosen to mimic physiological conditions, and the effects of FA–bovine serum albumin (BSA) complexes were tested in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Nitric oxide (NO) and various proteins/factors in RAW264.7 cells were quantified as follows: NO by the Griess assay; prostaglandin (PG) E2, interleukin (IL)-6 and tumor necrosis factor (TNF)-α by ELISA; inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2… CONTINUE READING
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