The linker region plays a key role in the adaptation to cold of the cellulase from an Antarctic bacterium.

@article{Sonan2007TheLR,
  title={The linker region plays a key role in the adaptation to cold of the cellulase from an Antarctic bacterium.},
  author={Guillaume K Sonan and V{\'e}ronique Receveur-Br{\'e}chot and Colette Duez and Nushin Aghajari and Mirjam Czjzek and Richard Haser and Charles Gerday},
  journal={The Biochemical journal},
  year={2007},
  volume={407 2},
  pages={
          293-302
        }
}
The psychrophilic cellulase, Cel5G, from the Antarctic bacterium Pseudoalteromonas haloplanktis is composed of a catalytic module (CM) joined to a carbohydrate-binding module (CBM) by an unusually long, extended and flexible linker region (LR) containing three loops closed by three disulfide bridges. To evaluate the possible role of this region in cold adaptation, the LR was sequentially shortened by protein engineering, successively deleting one and two loops of this module, whereas the last… 

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