The limitations and validities of senescence associated-β-galactosidase activity as an aging marker for human foreskin fibroblast Hs68 cells

@article{Yang2005TheLA,
  title={The limitations and validities of senescence associated-$\beta$-galactosidase activity as an aging marker for human foreskin fibroblast Hs68 cells},
  author={Nae-Cherng Yang and Miao-lin Hu},
  journal={Experimental Gerontology},
  year={2005},
  volume={40},
  pages={813-819}
}
  • N. Yang, M. Hu
  • Published 1 October 2005
  • Biology, Medicine
  • Experimental Gerontology
The senescence associated-beta-galactosidase (SA-betaG) assay has become one of the most commonly used markers of cell-aging. However, the reliability of the assay is questionable because the enzyme is a non-specific marker for cell-aging. In this study, we found that the SA-betaG activity increased with cell age as well as in confluent quiescent cells or cells under serum starvation, and in cells treated with H2O2. Importantly, we found that SA-betaG activity was irreversibly increased in the… Expand
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