The ligand affinity of proteins measured by isothermal denaturation kinetics.

@article{Epps2001TheLA,
  title={The ligand affinity of proteins measured by isothermal denaturation kinetics.},
  author={Dennis E. Van Epps and Ronald W Sarver and Joseph M. Rogers and John T Herberg and Paul K. Tomich},
  journal={Analytical biochemistry},
  year={2001},
  volume={292 1},
  pages={40-50}
}
An isothermal denaturation kinetic method was developed for identifying potential ligands of proteins and measuring their affinity. The method is suitable for finding ligands specific toward proteins of unknown function and for large-scale drug screening. It consists of analyzing the kinetics of isothermal denaturation of the protein-with and without the presence of potential specific ligands-as measured by long-wavelength fluorescent dyes whose quantum yield increases when bound to hydrophobic… CONTINUE READING

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