The latest advancements in proteomic two-dimensional gel electrophoresis analysis applied to biological samples.

Abstract

Two-dimensional gel electrophoresis (2DE) is one of the fundamental approaches in proteomics for the separation and visualization of complex protein mixtures. Proteins can be analyzed by 2DE using isoelectric focusing (IEF) in the first dimension, combined to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the second dimension, gel… (More)
DOI: 10.1007/978-1-4939-1872-0_6

Topics

Cite this paper

@article{Santucci2015TheLA, title={The latest advancements in proteomic two-dimensional gel electrophoresis analysis applied to biological samples.}, author={Laura Santucci and Maurizio Bruschi and Gian Marco Ghiggeri and Giovanni Candiano}, journal={Methods in molecular biology}, year={2015}, volume={1243}, pages={103-25} }