The last piece in the cellulase puzzle: the characterisation of β-glucosidase from the herbivorous gecarcinid land crab Gecarcoidea natalis

  title={The last piece in the cellulase puzzle: the characterisation of $\beta$-glucosidase from the herbivorous gecarcinid land crab Gecarcoidea natalis},
  author={Benjamin James Allardyce and Stuart M Linton and Reinhard Saborowski},
  journal={Journal of Experimental Biology},
  pages={2950 - 2957}
SUMMARY A 160 kDa enzyme with β-glucosidase activity was purified from the midgut gland of the land crab Gecarcoidea natalis. The enzyme was capable of releasing glucose progressively from cellobiose, cellotriose or cellotetraose. Although β-glucosidases (EC have some activity towards substrates longer than cellobiose, the enzyme was classified as a glucohydrolase (EC as it had a preference for larger substrates (cellobiose<cellotriose=cellotetraose). It was able to… Expand
Synergistic interaction of an endo-β-1,4-glucanase and a β-glucohydrolase leads to more efficient hydrolysis of cellulose-like polymers in the gecarcinid land crab, Gecarcoidea natalis
The synergistic interaction of these two enzymes further supports the previous suggestion that Gecarcoidea natalis possesses a novel two-enzyme cellulase system that differs from the traditional three-en enzyme fungal model. Expand
Identification and Characterization of 1,4-β-D-Glucan Glucohydrolase for the Saccharification of Cell Oligomers from Paenibacillus sp. HPL-001
The 1,4-β-D-glucan glucohydrolase gene (Ggh) which was isolated from Paenibacillus sp. strain HPL-001 (KCTC11365BP) has been cloned and expressed in Escherichia coli, and efficiently purified usingExpand
Isozymes from the herbivorous gecarcinid land crab, Gecarcoidea natalis that possess both lichenase and endo-β-1,4-glucanase activity.
  • S. Linton, Alicia J. Shirley
  • Biology, Medicine
  • Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
  • 2011
Three isozymes with both lichenase and endo-β-1,4-glucanase activity were purified and characterised from the midgut gland of the herbivorous gecarcinid land crab, Gecarcoidea natalis, suggesting that they may be able to hydrolyse hemicelluloses such as lichenan. Expand
Presence and activity of endo-β-1,4-mannase, an important digestive carbohydrase within the digestive fluid of terrestrial crustaceans.
  • S. Linton
  • Medicine
  • Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
  • 2021
High endo-β-1,4-mannase activities suggest that digestion of mannan within these species may represent an important source of carbohydrate. Expand
Characterisation of cellulose and hemicellulose digestion in land crabs with special reference to Gecarcoidea natalis
The evidence for the endogenous production of the cellulase and hemicellulase enzymes, their evolutionary origin and possible evolution in invertebrates as they colonised land is discussed. Expand
Comprehensive Enzymatic Analysis of the Cellulolytic System in Digestive Fluid of the Sea Hare Aplysia kurodai. Efficient Glucose Release from Sea Lettuce by Synergistic Action of 45 kDa Endoglucanase and 210 kDa ß-Glucosidase
It is indicated that 45K cellulase and 210K ß-glucosidase are the core components of the sea hare digestive system for efficient production of glucose from sea lettuce. Expand
Characterization of a novel thermostable β-glucosidase from a metagenomic library of termite gut.
Results suggest that the thermostable β-glucosidase Bgl-gs1 is a likely candidate for industrial applications. Expand
A Beta-glucosidase with Beta-xylosidase Activity from the Digestive Juice of the Land Crab Cardisoma armatum
A bifunctional beta-glucosidase/beta-xylosidase enzyme from land crab Cardisoma armatum was purified to homogeneity by anion-exchange, size-exclusion, cation-exchange and hydrophobic interactionExpand
Transcriptome-Guided Identification of Carbohydrate Active Enzymes (CAZy) from the Christmas Island Red Crab, Gecarcoidea natalis and a Vote for the Inclusion of Transcriptome-Derived Crustacean CAZys in Comparative Studies
A highly expressed transcript coding for GH5_10 previously missed by conventional screening of cellulase activity was inferred to be a novel endo-β-1,4-mannase in G. natalis, supporting endogenous expression and underscoring the utility of whole transcriptome in uncovering new CAZy-coding transcripts. Expand
Digestive enzymes of two brachyuran and two anomuran land crabs from Christmas Island, Indian Ocean
The digestive ability of four sympatric land crabs species was examined by determining the activity of their digestive enzymes, and the digestive fluid of C. perlatus possessed higher endo-β-1,4-glucanase, lichenase and laminarinase activities compared to that of the other species. Expand


Purification and characterisation of endo-β-1,4-glucanase and laminarinase enzymes from the gecarcinid land crab Gecarcoidea natalis and the aquatic crayfish Cherax destructor
Laminarinase and endo-β-1,4-glucanase were purified and characterised from the midgut gland of the herbivorous land crab Gecarcoidea natalis and the crayfish Cherax destructor and produced glucose and other short oligomers from the hydrolysis of laminarin. Expand
Cloning and Characterization of the Glucooligosaccharide Catabolic Pathway β-Glucan Glucohydrolase and Cellobiose Phosphorylase in the Marine HyperthermophileThermotoga neapolitana
ABSTRACT Characterization in Thermotoga neapolitana of a catabolic gene cluster encoding two glycosyl hydrolases, 1,4-β-d-glucan glucohydrolase (GghA) and cellobiose phosphorylase (CbpA), and theExpand
Characterization of a β-glycosidase highly active on disaccharides and of a β-galactosidase from Tenebrio molitor midgut lumen
The midgut of the yellow mealworm, Tenebrio molitor L. (Coleoptera: Tenebrionidae) larvae has four β-glycosidases. The properties of two of these enzymes (βGly1 and βGly2) have been describedExpand
Physical properties, substrate specificities and a probable mechanism for a β-d-glucosidase (cellobiase) from midgut cells of the cassava hornworm (Erinnyis ello)
Erinnyis ello midgut cells display a β-d-glucosidase (cellobiase, β-d-glucoside glucohydrolase EC with Mr 129 000 and pI 6.8. The enzyme hydrolyzes β-d-glucosides, β-d-galactosides andExpand
Purification and properties of a beta-glycosidase purified from midgut cells of Spodoptera frugiperda (Lepidoptera) larvae.
Two beta-glycosidases (BG) were purified from Spodoptera frugiperda (Lepidoptera: Noctuidae) midguts and the results suggest the presence of a carboxylate as a catalytic group. Expand
Purification and characterization of an endo-1, 4-β-D glucanase from the cellulolytic system of the wood-boring marine mollusk Lyrodus pedicellatus (Bivalvia: Teredinidae)
Results are consistent with the conclusion that Lp-egl-1 is an endo-1, 4-β-D glucanase, secreted by a cultivable endosymbiont of shipworms, Teredinibacter turnerae Distel et al., 2002. Expand
Endogenous production of endo-β-1,4-glucanase by decapod crustaceans
The potential ability to produce cellulase enzymes endogenously was examined in decapods crustaceans including the herbivorous gecarcinid land crabs Gecarcoideanatalis and Discoplax hirtipes, theExpand
Enzymatic studies on a cellulase system of Trichoderma viride. III. Transglycosylation properties of two cellulase components of random type.
Two highly purified cellulases, obtained from the cellulase system of Trichoderma viride, were found to catalyze the synthesis of cellotriose from rho-nitrophenyl beta-D-cellobioside by transfer of a glucosyl residue, possibly to cellobiose produced in the reaction mixture. Expand
Presence and properties of cellulase and hemicellulase enzymes of the gecarcinid land crabs Gecarcoidea natalis and Discoplax hirtipes
SUMMARY Digestive juice from the herbivorous gecarcinid land crabs Gecarcoidea natalis and Discoplax hirtipes exhibited total cellulase activity and activities of two cellulase enzymes;Expand
Purification, molecular cloning, and properties of a beta-glycosidase isolated from midgut lumen of Tenebrio molitor (Coleoptera) larvae.
Two beta-glycosidases were purified from midgut contents of larvae of the yellow mealworm, Tenebrio molitor, and the putative physiological role of these enzymes is the digestion of di- and oligosaccharides derived from hemicelluloses. Expand