The last piece in the cellulase puzzle: the characterisation of β-glucosidase from the herbivorous gecarcinid land crab Gecarcoidea natalis

@article{Allardyce2010TheLP,
  title={The last piece in the cellulase puzzle: the characterisation of $\beta$-glucosidase from the herbivorous gecarcinid land crab Gecarcoidea natalis},
  author={Benjamin James Allardyce and Stuart M Linton and Reinhard Saborowski},
  journal={Journal of Experimental Biology},
  year={2010},
  volume={213},
  pages={2950 - 2957}
}
SUMMARY A 160 kDa enzyme with β-glucosidase activity was purified from the midgut gland of the land crab Gecarcoidea natalis. The enzyme was capable of releasing glucose progressively from cellobiose, cellotriose or cellotetraose. Although β-glucosidases (EC 3.2.1.21) have some activity towards substrates longer than cellobiose, the enzyme was classified as a glucohydrolase (EC 3.2.1.74) as it had a preference for larger substrates (cellobiose<cellotriose=cellotetraose). It was able to… Expand
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