The last few milliseconds in the life of a secretory granule

@article{Oheim1998TheLF,
  title={The last few milliseconds in the life of a secretory granule},
  author={Martin Oheim and Dinah Loerke and Walter Stuehmer and Robert H. Chow},
  journal={European Biophysics Journal},
  year={1998},
  volume={27},
  pages={83-98}
}
We have monitored single vesicles (granules) in bovine adrenal chromaffin cells using an optical sectioning technique, total internal reflection fluorescence microscopy (TIRFM). With TIR, fluorescence excitation is limited to an optical slice near a glass/water interface. In cells located at the interface, granules loaded with fluorescent dye can be visualized near to or docked at the plasma membrane. Here we give evidence that (1) TIRFM resolves single vesicles and (2) the fluorescence signal… CONTINUE READING

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