The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells.

@article{Collins1995TheKO,
  title={The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells.},
  author={Andrew R Collins and Ai-guo Ma and Susan J. Duthie},
  journal={Mutation research},
  year={1995},
  volume={336 1},
  pages={69-77}
}
Single cell gel electrophoresis is a sensitive method for detecting DNA strand breaks. Cells embedded in agarose are converted to nucleoids by treating with detergent and high salt. DNA breaks render the nucleoid DNA susceptible to extension by electrophoresis, forming 'comets'. We find that when DNA breakage resulting from H2O2 treatment is examined, freshly isolated normal human lymphocytes are relatively resistant compared with transformed human cells. When incubated after treatment with… CONTINUE READING

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