The kinetics of methotrexate polyglutamation in human breast cancer cells.

@article{Morrison1987TheKO,
  title={The kinetics of methotrexate polyglutamation in human breast cancer cells.},
  author={Paul F. Morrison and Carmen Joseph Allegra},
  journal={Archives of biochemistry and biophysics},
  year={1987},
  volume={254 2},
  pages={597-610}
}
The polyglutamation kinetics of methotrexate (MTX) in MCF-7 human breast cancer cells have been formulated mathematically. The model takes account of glutamation and hydrolysis kinetics up through the pentaglutamate level, increased synthesis of dihydrofolate reductase following exposure to drug, reversible tight-binding to reductase, and membrane transport of polyglutamates. The glutamation, hydrolysis, and efflux parameters have been determined from fits to experimental MTX polyglutamate… CONTINUE READING

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