The kinetic mechanism of AAC3-IV aminoglycoside acetyltransferase from Escherichia coli.

@article{Magalhes2005TheKM,
  title={The kinetic mechanism of AAC3-IV aminoglycoside acetyltransferase from Escherichia coli.},
  author={Maria L B Magalh{\~a}es and John S Blanchard},
  journal={Biochemistry},
  year={2005},
  volume={44 49},
  pages={
          16275-83
        }
}
The aminoglycoside 3-N-acetyltransferase AAC(3)-IV from Escherichia coli exhibits a very broad aminoglycoside specificity, causing resistance to a large number of aminoglycosides, including the atypical veterinary antibiotic, apramycin. We report here on the characterization of the substrate specificity and kinetic mechanism of the acetyl transfer reaction catalyzed by AAC(3)-IV. The steady-state kinetic parameters revealed a narrow specificity for the acyl-donor and broad range of activity for… CONTINUE READING

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