The kinetic factors that determine the affinity and selectivity for slow binding inhibition of human prostaglandin H synthase 1 and 2 by indomethacin and flurbiprofen.

Abstract

We present here for the first time a method for determining the rate constants associated with slow binding inhibition of prostaglandin H synthase (PGHS). The rate constants were determined by a method using initial steady-state conditions, which minimize the impact of catalytic autoinactivation of the enzyme. The currently available methods for determining… (More)

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@article{Callan1996TheKF, title={The kinetic factors that determine the affinity and selectivity for slow binding inhibition of human prostaglandin H synthase 1 and 2 by indomethacin and flurbiprofen.}, author={Ondine Callan and Oldfield K. Y. So and David C. Swinney}, journal={The Journal of biological chemistry}, year={1996}, volume={271 7}, pages={3548-54} }