The isolation of mutants not accumulating poly-β-hydroxybutyric acid

@article{Schlegel2004TheIO,
  title={The isolation of mutants not accumulating poly-$\beta$-hydroxybutyric acid},
  author={Hans G{\"u}nter Schlegel and R. M. Lafferty and I. Krauss},
  journal={Archiv f{\"u}r Mikrobiologie},
  year={2004},
  volume={71},
  pages={283-294}
}
SummaryFive mutant strains of Hydrogenomonas H 16 which synthesize poly-β-hydroxybutyric acid either slowly or not at all have been isolated following nitrite and NMG treatment of wild type cells. When grown on a nitrogen deficient agar medium, the colonies of PHB-free cells can be recognized by their diminished retention of the dye sudanblack B. Enrichment procedures for such mutants have been devised employing the 32P-phosphate inactivation technique and sucrose gradient centrifugation. The… Expand

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