The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme.

@article{Wolfe1982TheIO,
  title={The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme.},
  author={Peter Wolfe and Paula Silver and William Wickner},
  journal={The Journal of biological chemistry},
  year={1982},
  volume={257 13},
  pages={7898-902}
}
Leader (signal) peptidases cleave the NH2-terminal leader sequences of newly synthesized secreted and membrane proteins during, or shortly after, they insert across the membrane. We have constructed a plasmid, pPS9, in which the structural gene for Escherichia coli leader peptidase is under transcriptional control of a lambda promoter, PR. pPS9 also codes for a temperature-sensitive lambda repressor, causing repression of expression of the plasmid leader peptidase gene at 30 degrees C and rapid… CONTINUE READING