The isolation of dicyclohexylcarbodi-imide-binding proteins from mitochondrial membranes.

  title={The isolation of dicyclohexylcarbodi-imide-binding proteins from mitochondrial membranes.},
  author={Kenneth J. Cattell and I. G. Knight and Christine Lindop and R. Brian Beechey},
  journal={The Biochemical journal},
  volume={117 5},
DCCD* is a potent inhibitor of oxidative phosphorylation. It has been suggested that its site of action may be an intermediate of ATP synthesis with which it forms a covalent bond (Beechey, Roberton, Holloway & Knight, 1967). Therefore it is desirable to isolate and characterize the component(s) in the mitochondrion with which DCCD interacts. Knight, Holloway, Roberton & Beechey (1968) have shown that the radioactivity in [14C]DCCD-treated mitochondria or submitochondrial particles was… 
37 Citations
Inheritance of Oligomycin Resistance in Tissue Culture Cells
The mitochondrial ATPase complex has been resolved into the soluble F1, having the catalytic site for ATP synthesis, and the hydrophobic membrane-integrated F0, involved in proton translocation
Inhibitory action of carbodiimides on bacterial membrane ATPase.
  • A. Abrams, C. Baron
  • Chemistry, Biology
    Biochemical and biophysical research communications
  • 1970
Identification of the subunits of F1F0-ATPase from bovine heart mitochondria.
An oligomycin-sensitive F1F0-ATPase isolated from bovine heart mitochondria has been reconstituted into phospholipid vesicles and pumps protons, which is more complex than bacterial and chloroplast enzymes, and contains 14 different polypeptides that are resolved by polyacrylamide gel electrophoresis under denaturing conditions.
Synthesis of proteolipid protein by yeast mitochondria.
  • D. Murray, A. Linnane
  • Biology, Chemistry
    Biochemical and biophysical research communications
  • 1972
Photoaffinity labeling of uncoupler binding sites on mitochondrial membrane
Abstract3H 2-azido-4-nitrophenol, a photoactive uncoupler, has been synthesized, and its uncoupling action on oxidative phosphorylation and its binding to the mitochondrial membrane have been