The isolation of an acetylcholine- and decamethonium-binding protein from housefly heads.

@article{Cattell1972TheIO,
  title={The isolation of an acetylcholine- and decamethonium-binding protein from housefly heads.},
  author={Kenneth J. Cattell and John F. Donnellan},
  journal={The Biochemical journal},
  year={1972},
  volume={128 1},
  pages={
          187-9
        }
}
Protein fractions thought to contain an acetylcholine-receptor molecule have been isolated from a variety ofnervous tissues. The experimental approach used by Miledi &Potter (1971) and Miledi etal. (1971) has been to label receptor molecules in the electric organs of fish or vertebrate muscle preparations with radioiodinated a-bungarotoxin in order to facilitate their isolation by fractionation techniques involving the use of detergent solutions. De Robertis and his co-workers (De Robertis… 

Figures from this paper

ISOLATION AND PURIFICATION OF CHOLINERGIC RECEPTOR PROTEOLIPIDS FROM RAT GASTROCNEMIUS TISSUE 1
  • R. F. Taylor
  • Biology, Chemistry
    Journal of neurochemistry
  • 1978
TLDR
The purified receptor proteolipid resulting from wet tissue extraction has been characterized as a somatic, nicotinic cholinergic receptor by its specificity and binding kinetics toward a variety of drugs and toxins.
SUBCELLULAR LOCALIZATION AND PROPERTIES OF A CHOLINERGIC RECEPTOR ISOLATED FROM HOUSEFLY HEADS
—Protein(s) possessing the ligand‐binding properties expected of the insect cholinergic receptor were demonstrated in both aqueous and chloroform‐methanol extracts of an 80,000 g supernatant obtained
Actions of cholinergic pharmacological agents on the cell body membrane of the fast coxal depressor motoneurone of the cockroach (Periplaneta americana)
TLDR
Of the three putative receptor-specific ligands used to date in binding studies on insect CNS tissues, α-bungarotoxin (α-BGTX) was much more effective in blocking the depolarization resulting from ionophoretic application of ACh, than either quinuclidinyl benzilate (QNB) or decamethonium (I 50 × 2.8×10 −3 M).
Studies on the nicotinic acetylcholine receptor of the locust
General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing
Synaptic Transmission in Insects
TLDR
Insects have never attracted the attentions of biochemists to the same extent as either microorganisms or vertebrates, but a major part of their current knowledge of biochemistry stems from observations using either tissue from the rat or E. coli.
...
1
2
...