The isolation of acrosome-reaction-inducing glycoproteins from sea urchin egg jelly.

@article{Keller1994TheIO,
  title={The isolation of acrosome-reaction-inducing glycoproteins from sea urchin egg jelly.},
  author={Steven H. Keller and Victor D. Vacquier},
  journal={Developmental biology},
  year={1994},
  volume={162 1},
  pages={
          304-12
        }
}
The solubilized jelly coat of Strongylocentrotus purpuratus eggs was boiled in SDS and mercaptoethanol, precipitated in ethanol, washed in 70% ethanol, lyophilized, and redissolved in seawater. The sample retained its activity to induce the sperm acrosome reaction (AR), showing that the AR inducer was resistant to harsh denaturing conditions. Egg jelly (EJ) was separated into its component macromolecules by denaturing polyacrylamide gel electrophoresis and the gel silver-stained. Several… CONTINUE READING

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