The isolation of a Dol-P-Man synthase from Ustilago maydis that functions in Saccharomyces cerevisiae.

@article{Zimmerman1996TheIO,
  title={The isolation of a Dol-P-Man synthase from Ustilago maydis that functions in Saccharomyces cerevisiae.},
  author={John W. Zimmerman and Charles A. Specht and B X Cazares and Phillips W. Robbins},
  journal={Yeast},
  year={1996},
  volume={12 8},
  pages={765-71}
}
Genomic DNAs from several fungi were screened for a homologous sequence to Saccharomyces cerevisiae DPM1, an essential gene which encodes dolichyl phosphoryl mannose synthase. The fungi examined included Aspergillus nidulans, Neurospora crassa, Schizophyllum commune and Ustilago maydis. Only U. maydis gave a significant signal after Southern hybridization using DPM1 as a probe. The Ustilago homolog was subsequently cloned and sequenced. The predicted protein of 294 amino acids has 60% identity… CONTINUE READING