The isolation and preliminary characterization of N-acetyl-D-glucosamine kinase from rat kidney and liver.

@article{Allen1980TheIA,
  title={The isolation and preliminary characterization of N-acetyl-D-glucosamine kinase from rat kidney and liver.},
  author={Mary Belle Allen and Deryck G. Walker},
  journal={The Biochemical journal},
  year={1980},
  volume={185 3},
  pages={565-75}
}
1. Procedures for the extensive purification in high yield of N-acetyl-D-glucosamine kinase from rat liver and kidney are described. The separation of this enzyme from hepatic glucokinase depended primarily on their differing behaviour on an affinity column of Sepharose--N-(6-aminohexanoyl)-2-amino-2-deoxy-D-glucopyranose. 2. This N-acetyl-D-glucosamine kinase also catalyses the phosphorylation of N-acetyl-D-mannosamine and, at a lower rate, several other sugar analogues, including D-glucose. 3… CONTINUE READING