The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.

@article{Smith1976TheIA,
  title={The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.},
  author={David I. Smith and Frank R Blattner and J. W. L. Davies},
  journal={Nucleic acids research},
  year={1976},
  volume={3 2},
  pages={
          343-53
        }
}
We describe the isolation of a new class 2 restriction endonuclease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and requirements. Pst 1 requires Mg++ as the only cofactor and produces more than 18 cleavages in wild type lambda. We have determined the location of 7 of these cleavages by the use of deletion and insertion mutants of lambda. 
The Mapping and sequence determination of the single site in φX174am3 replicative form DNA cleaved by restriction endonuclease Pst I
TLDR
The mapping of the single Pst I site in ~bX174am3 RFI DNA and the determination of the sequence cleaved is described and this is the symmetrical hexanucleotide sequence. Expand
Two restriction-like enzymes from Xanthomonas malvacearum.
Two sequence-specific endonucleases, Xma I and Xma II, have been purified from Xanthomonas malvacearum . Xma I makes cuts in bacteriophage lambda and adenovirus-2 DNA identical with those produced byExpand
Cell localization of EcoRI endonuclease in Escherichia coli K-12
TLDR
Results showed that EcoRI activity was almost entirely recovered into cytoplasmic fractions and consequently was not released into the extracellular medium by a tolA mutant, which did not support previous reports suggesting a periplasmic location for the EcoRI enzyme and did not allow for a simple method for EcoRI purification from culture supernatants of excretory mutants. Expand
Dye-ligand chromatography for the resolution and purification of restriction endonucleases
TLDR
A three-step Chromatographic procedure has been developed to purify EcoRV suitable for commercial exploitation, as judged by the “overdigestion” and “cut-ligate-recut” quality control tests. Expand
Widespread occurrence of the restriction endonuclease YenI, an isoschizomer of PstI, in Yersinia enterocolitica serotype O8
The cold-active restriction endonuclease YenI, an isoschizomer of PstI, was found in 12 of 14 Yersinia enterocolitica serotype O8 strains of different origins, but not in other serotypes of Y.Expand
Location of the cleavage sites on the SV 40 DNA map produced by the restriction endonucleases Pst1 and Bam1
  • I. Fodor
  • Biology, Medicine
  • Molecular Biology Reports
  • 2004
Restriction endonucleases from Providencia stuartii (Pst 1) and Bacillus amyloliquefaciens H (Bam 1) cleave SV 40 DNA at two and one specific sites, respectively. Using EcoRI and Hind IIIExpand
A simple and rapid method for screening bacteria for type II restriction endonucleases: enzymes in Aphanothece halophytica
A method is described which allows a large number of bacterial strains to be rapidly and easily screened for the presence of site-specific endonucleases. The method involves selectiveExpand
Mapping of restriction sites in the attachment site region of bacteriophage lambda
TLDR
A fine structure map of the EcoRI fragment containing the lambda attachment-site region has been constructed and complete cleavage maps of the entire lambda genome have been obtained for endonucleases BglII,BluI,KpnI, SacI,SacII,SalI andXbaI. Expand
Investigation of sequence homology in a group of type-II restriction/modification isoschizomers.
TLDR
Using computer similarity searches to look for homology between the PstI proteins and the known sequences of other type-II systems that recognise different sites, a possible recognition domain within the M.PstI methyltransferase is postulate based on similarity to theM.PaeR7 and M.TaqI methyl transferases. Expand
Restriction enzyme cleavage map of Tn10, a transposon which encodes tetracycline resistance.
TLDR
A cleavage map of a recombinant plasmid carrying Tn10 was constructed for 13 different restriction enzymes; it confirmed the previously reported structure of this transposon, which in part codes for the tetracycline resistance functions and is bounded by inverted repeats. Expand
...
1
2
3
4
5
...