The isolation and partial characterization of a herpesvirus from a case of bovine metritis

  title={The isolation and partial characterization of a herpesvirus from a case of bovine metritis},
  author={J B Parks and John W. Kendrick},
  journal={Archiv f{\"u}r die gesamte Virusforschung},
A virus isolated from the uterine exudate of a cow with metritis had physical, chemical and cytopathogenic properties characteristic of a member of the herpesvirus group. Neutralization tests indicated that the virus was not related to any of the previously described bovine herpesviruses. 
Twelve slow-growing virus isolates propagated in tissue culture from cattle with epididymitis and vaginitis were identified as herpes viruses by virtue of their biological, physicochemical and morphological characteristics.
Characterization of a persistent infection with bovine herpesvirus type-4
The usefulness of the rabbit as a model for studying the pathogenesis of BHV-4 infections in cattle is demonstrated, with high titer levels that persisted throughout the experiment.
First Serbian isolates of bovine herpesvirus 4 (BoHV-4) from a herd with a history of postpartum metritis.
This was the first isolation and characterisation of BoHV4 from Serbian herds and two isolates showed similarity with MOVAR 33/63 virus type, but one differed in polyrepetitive and other parts of DNA.
Bovine Herpesvirus 4 in Parana State, Brazil: case report, viral isolation, and molecular identification
From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches, demonstrating that agent was a bovine herpesvirus 4, appertaining to DN-599 group.
Isolation and characterization of bovine herpesvirus 4 (BoHV-4) from a cow affected by post partum metritis and cloning of the genome as a bacterial artificial chromosome
This study aimed to develop a tool enabling precise genetic discrimination between strains of BoHV-4 and to easily manipulate the viral genome to explore the mechanistic role of viral strain variation for uterine disease.
Virological and serological evidence of bovine herpesvirus type 4 in cattle in Northern Ireland
Serological testing failed to demonstrate a significant association between the exposure of the cattle to BHV-4 and the metritis, but several cattle seroconverted during the periparturient period, consistent with the recrudescence and shedding of virus associated with the stresses of parturition and the onset of lactation.
BHV4 (bovine herpes virus 4) related disorders in Belgian cattle: A study of two problem herds
Two cattle farms, with a ten year history of BHV4 related postpartum metritis accompanied by fertility problems, were monitored during the winter season 1985–1986 and a possible role of BhV4 in the respiratory problems observed during the study is suggested.
Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function.
Evidence of cellular and molecular mechanisms are provided, supporting the concept that BoHV-4 is a pathogen associated with uterine disease.
Bovine Endometrial Stromal Cells Support Tumor Necrosis Factor Alpha-Induced Bovine Herpesvirus Type 4 Enhanced Replication1
Light is shed on two important and rather controversial issues: the role of TNF-alpha receptor, which is weakly expressed in the stromal layer of the bovine uterus, as well as the possible interactions between proinflammatory molecules, viral replication, and chronic uterine disease.
Primary replication and invasion of the bovine gammaherpesvirus BoHV-4 in the genital mucosae
In this study, in vivo-related in vitro genital tract models were successfully established and the early stage of the pathogenesis of a genital infection was clarified: BoHV-4 starts with a productive infection of epithelial cells in the reproductive tract, forming small foci followed by a non-productive infection of surveilling monocytic cells which help BoHv-4 to invade into deeper tissues.


A new bovine herpesvirus and its effect on experimentally infected calves
The isolation of a new bovine herpesvirus is described and its effect on experimentally infected calves is described to study the pathogenicity of the agent.
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