The isolation and characterization of Ca++-accumulating subcellular membrane fractions from cerebral arteries.

@article{Barry1985TheIA,
  title={The isolation and characterization of Ca++-accumulating subcellular membrane fractions from cerebral arteries.},
  author={K. J. Barry and R. Mikkelsen and W. Shucart and E. Keough and V. Gavris},
  journal={Journal of neurosurgery},
  year={1985},
  volume={62 5},
  pages={
          729-36
        }
}
A study was undertaken using differential centrifugation methods to isolate from rabbit cerebral arteries the subcellular microsomal protein fractions capable of actively sequestering Ca++. One isolated protein fraction displayed a relatively large adenosine triphosphate (ATP)-dependent Ca++-accumulating capacity that was completely inhibited by NaN3, and was therefore designated the "mitochondrial fraction." Electron microscopy confirmed that this fraction consisted of numerous mitochondrial… Expand
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