The isolation and characterization of a Norwalk virus-specific cDNA.

  title={The isolation and characterization of a Norwalk virus-specific cDNA.},
  author={Suzanne M Matsui and Jung-suh P. Kim and Harry B Greenberg and W Su and Qing Sun and Philip C. Johnson and H L DuPont and L. S. Oshiro and Gregory R. Reyes},
  journal={The Journal of clinical investigation},
  volume={87 4},
Norwalk virus, an important cause of epidemic, acute, nonbacterial gastroenteritis in adults and children, has eluded adaptation to tissue culture, the development of an animal model, and molecular cloning. In this study, a portion of the Norwalk viral genome encoding an immunoreactive region was cloned from very small quantities of infected stool using sequence-independent single primer amplification. Six overlapping complementary DNA (cDNA) clones were isolated by immunologic screening. The… CONTINUE READING


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