The isolation and characterisation of a major outer-membrane protein from Bacteroides distasonis.

@article{Wexler1992TheIA,
  title={The isolation and characterisation of a major outer-membrane protein from Bacteroides distasonis.},
  author={Hannah M. Wexler and Cindy Getty and Graham Fisher},
  journal={Journal of medical microbiology},
  year={1992},
  volume={37 3},
  pages={165-75}
}
An outer-membrane protein (OMP) was isolated from a clinical strain of Bacteroides distasonis. Changes in growth media did not appreciably affect the appearance of this protein in crude outer-membrane preparations examined by SDS-PAGE. However, the proportion of the protein relative to other OMPs was greater in 24-h cultures than in 48-h cultures. The protein could not be readily solubilised by various conventional detergent extraction techniques but treatment of the insoluble material at 100… CONTINUE READING

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