The isolation, characterisation, and chromosomal assignment of the gene for human 3-hydroxy-3-methylglutaryl coenzyme A reductase, (HMG-CoA reductase)

  title={The isolation, characterisation, and chromosomal assignment of the gene for human 3-hydroxy-3-methylglutaryl coenzyme A reductase, (HMG-CoA reductase)},
  author={Steve E. Humphries and F. Tata and Isabelle Henry and Fernande Barichard and M. Holm and Claudine Junien and R. Williamson},
  journal={Human Genetics},
SummaryWe have used a cDNA clone for Chinese hamster 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase to isolate a genomic recombinant for human HMG-CoA reductase. The identity of the gene was confirmed by partial sequence analysis. Several unique fragments that will be useful for restriction fragment length polymorphism (RFLP) studies were identified. In situ hybridisation of a 2.6kb unique fragment of the gene to human metaphase chromosomes localised the human HMGCoA reductase gene… 
Two novel SNPs of the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene associated with growth and meat quality traits in the chicken.
Based on association analysis of these HMGCR polymorphisms in 864 Gushi/Anka F(2) hybrids, these two mutations have significant effects on growth, carcass, meat quality, and lipid concentration.
SNP in intron 5 of 3-hydroxy-3-methylglutaryl coenzyme A reductase ( HMGR ) gene and its genetic effects on important economic traits in geese
The intron 5 of the 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) gene was cloned and its SNP was tested by PCR-SSCP in Wanxi White goose (WW), and the effect of genotype AB was contrary comparing with genotype AA.
Expression of HMGR in Lilu cattle tissues
HMGR mRNA expression is difference in adipose and muscle tissues suggesting this gene is expressed in a tissue-dependent manner in cattle, and understanding the causes of variation in HMGR gene expression may provide crucial information about cholesterol biosynthesis in Lilu beef cattle.
Expression of HMGR and corresponding cholesterol content in tissues of two pig breeds.
Results from this study indicate that cholesterol content and HMGR mRNA expression are higher in Huai pig tissues suggesting this gene is expressed in a breed- and tissue-dependent manner in pigs.
HMGR messenger RNA and protein expression in liver, kidney and muscle in pigs of two breeds.
The results demonstrate that HMGR expression in pigs may depend on tissue and species and the level of HMGR protein expression in liver was higher than in the muscle and renal tissues.
Natural resistance to infection with Legionella pneumophila: chromosomal localization of the Lgn1 susceptibility gene
Understanding the molecular basis of the high permissiveness of A/J macrophage to L. pneumophila may shed light on the survival strategy of this bacterium in highly permissive human phagocytes, and the identification of the Lgn1 subchromosomal region reported here is a first step towards that goal.
Therapeutic targets of hypercholesterolemia: HMGCR and LDLR
The regulatory mechanisms of HMGCR and LDLR are summarized to provide insight for new drug design and development in cholesterol homeostasis.
DMSO_A_219013 1543..1553
1Department of Endocrinology and Metabolism, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, People’s Republic of China; 2Department of Endocrinology and


Molecular cloning of 3-hydroxy-3-methylglutaryl coenzyme a reductase and evidence for regulation of its mRNA.
Data indicate that the primary translation product of reductase mRNA is a 90,000-dalton protein and that LDL suppresses the reduct enzyme in UT-1 cells by drastically reducing the level of its mRNA.
Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its mRNA in rat liver as studied with a monoclonal antibody and a cDNA probe.
It is concluded that treatment with cholestyramine and mevinolin increases the amount of reductase protein in rat liver by elevating the level of its mRNA and that cholesterol feeding to such induced rats lowers the amount.
The structural gene for aldolase B (ALDB) maps to 9q13→32
We used a cloned cDNA probe for the B subunit of human aldolase (ALDB) and Southern blotting techniques to analyse DNA from a series of rodent x human somatic cell hybrids for the presence of
Nucleotide sequence of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, a glycoprotein of endoplasmic reticulum
The nucleotide sequence of a 4.8-kilobase mRNA for hamster 3-hydroxy-3-methylglutaryl coenzyme A reductase, the endoplasmic reticulum enzyme that controls cholesterol biosynthesis, shows that it is a
The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.
The two independently isolated beta-globin clones differ from each other by the presence of a Pst I restriction enzyme cleavage site within the large intervening sequence of the delta- globin gene of one of the clones, which suggests that the human DNA carried in the two clones was derived from two homologous chromosomes which were heterozygous for the PstI restriction enzyme recognition sequence.
The isolation of a genomic clone containing the apolipoprotein CII gene and the detection of linkage disequilibrium between two common DNA polymorphisms around the gene
The usefulness of the apolipoprotein CII gene as a marker for linkage studies is increased by the use of both RFLPs, and marked linkage disequilibrium is observed between this RFLP and that detected with the apo CII cDNA clone.
Appearance of crystalloid endoplasmic reticulum in compactin-resistant Chinese hamster cells with a 500-fold increase in 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
A line of Chinese hamster ovary cells with a 500-fold increase in 3-hydroxy-3-methylglutaryl-coenzyme A reductase, the membrane-bound enzyme that controls cholesterol synthesis and also for studies of the synthesis and degradation of smooth ER is developed.
Multivalent feedback regulation of HMG CoA reductase, a control mechanism coordinating isoprenoid synthesis and cell growth.
The availability of compactin (ML-236B), a potent competitive inhibitor of 3-hydroxy-3-methylglutaryl Coenzyme A reductase, has permitted the demonstration of a hitherto unsuspected aspect of
Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent.
  • A. Alberts, J. Chen, +17 authors J. Springer
  • Chemistry, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1980
It was shown that mevinolin was an orally active cholesterol-lowering agent in the dog and orally administered sodium mevinolinate was an active inhibitor of cholesterol synthesis in an acute assay.
Chromosomal localization of the human apoprotein CI gene and of a polymorphic apoprotein AII gene.
Comparison of human and mouse chromosome 1 reveals a conserved group including apo AII, renin and peptidase genes and suggests that APOA2 will be found distal to this group on human chromosome 1.