The intracellular concentration of pyrophosphate in the batch culture of Escherichia coli.

@article{Kukko1982TheIC,
  title={The intracellular concentration of pyrophosphate in the batch culture of Escherichia coli.},
  author={E. I. Kukko and Jukka Heinonen},
  journal={European journal of biochemistry},
  year={1982},
  volume={127 2},
  pages={
          347-9
        }
}
We applied our colorimetric PPi determination method [Heinonen, J., Honkasalo, S. and Kukko, E. (1981) Anal. Biochem. 117, 293-300] to bacterial cultures and measured the intracellular concentration of PPi in the batch culture of Escherichia coli. The cells growing in minimal medium contained about 2.5 nmol PPi/mg protein (0.5 mM). There was no extracellular PPi. A similar concentration of PPi was found also in cells growing in minimal medium enriched by amino acid mixture. 
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Biological Role of Inorganic Pyrophosphate

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