The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies.

@article{Visintin2002TheIA,
  title={The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies.},
  author={Michela Visintin and Giovanni Settanni and Amos Maritan and Sergio Graziosi and James D Marks and Antonino Cattaneo},
  journal={Journal of molecular biology},
  year={2002},
  volume={317 1},
  pages={73-83}
}
We describe the application of an intracellular antibody capture technology (IACT) as a generic in vivo selection procedure for isolating intracellular antibodies or ICAbs. IACT was applied to the de novo selection of functional ICAbs against the microtubule-associated protein TAU, found in neurofibrillary lesions of Alzheimer's disease brains. A panel of 17 different ICAbs was created which bind TAU inside cells and the epitopes recognized by the selected ICAbs have been determined by an in… CONTINUE READING

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