The intracellular accumulation of UDP-N-acetylhexosamines is concomitant with the inability of human colon cancer cells to differentiate.

@article{Wice1985TheIA,
  title={The intracellular accumulation of UDP-N-acetylhexosamines is concomitant with the inability of human colon cancer cells to differentiate.},
  author={Burton M Wice and Germain Trugnan and Martha L. Pinto and Monique Rousset and Genevi{\`e}ve Chevalier and Elisabeth Dussaulx and Brigitte Lacroix and Alain Zweibaum},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 1},
  pages={139-46}
}
The relationship between the intracellular concentration of various nucleotides as measured by high-performance liquid chromatography analysis, and the differentiation of 2 human colon cancer cell lines was studied. HT-29 cells were induced to undergo both structural and functional enterocytic differentiation (as determined by electron microscopy and the presence of brush-border specific enzymes, respectively) by changing the carbon source or adding Na butyrate to standard tissue culture media… CONTINUE READING

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In all culture conditions where HT-29 cells did not differentite , the intracellular concentrations of 2 compounds which co - migrated with UDP - N - acetylglucosamine and UDP - N - acetylgalactosamine rose approximately equal to 10-fold at confluency and remained elevated throughout the stationary phase , whereas their concentrations remained constant and low after confluency in cells that underwent differentiation .
In all culture conditions where HT-29 cells did not differentite , the intracellular concentrations of 2 compounds which co - migrated with UDP - N - acetylglucosamine and UDP - N - acetylgalactosamine rose approximately equal to 10-fold at confluency and remained elevated throughout the stationary phase , whereas their concentrations remained constant and low after confluency in cells that underwent differentiation .
In all culture conditions where HT-29 cells did not differentite , the intracellular concentrations of 2 compounds which co - migrated with UDP - N - acetylglucosamine and UDP - N - acetylgalactosamine rose approximately equal to 10-fold at confluency and remained elevated throughout the stationary phase , whereas their concentrations remained constant and low after confluency in cells that underwent differentiation .
In all culture conditions where HT-29 cells did not differentite , the intracellular concentrations of 2 compounds which co - migrated with UDP - N - acetylglucosamine and UDP - N - acetylgalactosamine rose approximately equal to 10-fold at confluency and remained elevated throughout the stationary phase , whereas their concentrations remained constant and low after confluency in cells that underwent differentiation .
FructoseNo subtypeGlucose
Cells cultured in 25 mM fructose or glucose + /-
GlucoseNo subtypeFructose
Cells cultured in 25 mM fructose or glucose + /-
This differentiation occurred after the cells reached confluency when they were cultured in galactose , uridine , inosine , or without nucleosides ( all in the absence of glucose ) and in the presence of glucose plus Na butyrate .
This differentiation occurred after the cells reached confluency when they were cultured in galactose , uridine , inosine , or without nucleosides ( all in the absence of glucose ) and in the presence of glucose plus Na butyrate .
This differentiation occurred after the cells reached confluency when they were cultured in galactose , uridine , inosine , or without nucleosides ( all in the absence of glucose ) and in the presence of glucose plus Na butyrate .
This differentiation occurred after the cells reached confluency when they were cultured in galactose , uridine , inosine , or without nucleosides ( all in the absence of glucose ) and in the presence of glucose plus Na butyrate .
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