The interleukin 1 receptor. Dynamics of interleukin 1 binding and internalization in T cells and fibroblasts.

Abstract

In this study, we have demonstrated that a murine T cell lymphoma, EL 4, and a murine fibroblast cell line, Swiss 3T3, possess a single class of high affinity interleukin 1 (IL 1) receptors that exist in a dynamic state of equilibrium that is influenced by IL 1. In the absence of IL 1, the IL 1 receptor appears to turnover with a t1/2 of approximately 11 hr. However, when cells are incubated in the presence of IL 1, the IL 1 receptor undergoes extensive ligand-induced down-regulation. IL 1 itself is internalized at 37 degrees C; 50% of the surface-bound IL 1 is internalized in 60 to 120 min. IL 1 does not undergo degradation for at least 6 hr after internalization. By using electron microscopy and autoradiography, we observed several important features of the internalization process. When cells having bound 125I-IL 1 at 4 degrees C were shifted to 37 degrees C, IL 1 moved from the cell membrane to the cytoplasm where it was found in proximity to nuclei or within lysosomes. IL 1 appeared to progressively accumulate in nuclei. Six hours after shifting cells to 37 degrees C, 30 to 35% of the internalized 125I-IL 1 is associated with the cell nucleus. The accumulation of relatively high levels of IL 1 in the nucleus raises the interesting possibility that IL 1 may not only interact in a highly specific manner with cell surface receptors, but also with potentially important nuclear receptors.

050'90'93'96'99'02'05'08'11'14'17
Citations per Year

489 Citations

Semantic Scholar estimates that this publication has 489 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Mizel1987TheI1, title={The interleukin 1 receptor. Dynamics of interleukin 1 binding and internalization in T cells and fibroblasts.}, author={Steven B. Mizel and Patricia L. Kilian and Jon Lewis and Kathleen Paganelli and Richard A . Chizzonite}, journal={Journal of immunology}, year={1987}, volume={138 9}, pages={2906-12} }